Microscopic observations were easily misleading as a starch sheath often encloses pyrenoids.
2.
A starch sheath is often formed or deposited at the periphery of pyrenoids, even when that starch is synthesised in the cytosol rather than in the chloroplast.
3.
In " Chlamydomonas ", a high-molecular weight complex of two proteins ( LCIB / LCIC ) forms an additional concentric layer around the pyrenoid, outside the starch sheath, and this is currently hypothesised to act as a barrier to CO 2-leakage or to recapture CO 2 that escapes from the pyrenoid.
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